167691
Protocadherin-17 (PCDH17, PCDH68, PCH68)
Size
£825.00
- SKU:
- 167691
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa18-705 from human Protocadherin-17 expressed in human embryonic kidney cell line HEK293 (O14917).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Protocadherin 17 (PCDH-17) is a 120-150kD glycoprotein, d2-group member of the of the protocadherin subfamily, cadherin family of molecules. It is expressed on endothelium and epithelium such as stratified squamous epithelium and (likely) gastric columnar epithelium. PCDH17 is posited to act as an adhesion molecule, and appears to play a role in promoting cell cycle arrest and inhibiting migration. Mature human PCDH-17 is a type I transmembrane protein that is 1142aa in length. It contains a 690aa extracellular domain (ECD) (aa18-707) plus a 431aa cytoplasmic region. There are 6 cadherin domains in the ECD (aa18695). Two splice forms are reported that involve substitutions in the cytoplasmic region. The first possesses a 14aa substitution, while the second shows a 25aa substitution, both for aa876-1159. Over aa18-705, human PCDH-17 shares aa98% identity with mouse PCDH-17. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice