167688
Oligodendrocyte Marker O4 (APC)
Size
£1,010.00
- SKU:
- 167688
- Application:
- Flow Cytometry
- Purity:
- Purified by immunoaffinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine brain corpus callosum white matter
- Clone:
- O4
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.5% BSA and 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with bovine brain corpus callosum white matter. The IgG fraction of the tissue culture supernatant was purified by Protein A or G affinity chromatography. The purified antibody was then conjugated to APC fluorochrome. Cell surface expression of Oligodendrocyte Marker O4 is determined by flow cytometry using 620-650 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 660-670 nm. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 cells in differentiated rat cortical stem cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice