NUDT5 (ADP-Sugar Pyrophosphatase, Nudix motif 5, YSA1, YSA1H)

Size

£850.00

SKU:: 167687
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to aa2-219 from human ADP-Sugar Pyrophosphatase/NUDT5 expressed in E.coli (Q9UKK9).
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: ADP-Sugar Pyrophosphatase (NUDT5, Nucleoside diphosphate-linked moiety X motif 5; also YSA1H and 8-oxo-dGDP phosphatase) is a member of the Nudix hydrolase family of proteins. Although the predicted MW of NUDT-5 is 24kD, it runs anomalously at 32-36kD in SDS-Page. NUDT-5 is a widely expressed intracellular homodimer that has at least two functions. First, it possesses pyrophosphatase activity that prevents nonenzymatic ribosylation of proteins, and second, it hydrolyzes 8-oxoGDP (plus 8-OH-dADP), thus removing an oxidized nucleoside from the nucleotide pool and its potential for incorporation into either DNA or RNA. Such incorporation likely results in transcriptional or translational errors. Human NUDT-5 is 219aa in length (SwissProt #Q9UKK9). It is contains an N-terminal substrate binding region (aa28-51), a Nudix hydrolase domain (aa57-197) and an extended C-terminal a-helix (aa211-219) NUDT-5 is acetylated on Met1, Lys42, Lys210, and Lys218, and phosphorylated on Tyr74. There are two isoform variants, one that shows a deletion of aa163-165, and another that contains a 13aa substitution for aa164-219. Fulllength human and mouse NUDT-5 share 82%aa sequence identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice