NG2 (Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan, AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG) (PerCP)

Size

£806.00

SKU:: 167686
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human melanoma cells
Clone:: LHM-2
Format:: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with PerCP.
Extra Details:: NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma and glial precursor cells. NG2 also promotes epidermal stem cell patterned clustering. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells. Tested using G361 human melanoma cell line. Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.