Ly6K (Lymphocyte Antigen 6 Complex Locus K, CO16, CT97) (APC)

Size

£819.00

SKU:: 167678
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Gly138 from human Ly6K expressed in CHO cells.
Clone:: 750747
Format:: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: Ly6K (Lymphocyte antigen 6 locus K; also known as CO16) is a 26-27kD member of the Ly6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor patient blood. Mature human Ly6K is a 121aa GPI-linked (presumed) glycoprotein. It is synthesized as a 165aa pre-pro-precursor that contains a 17aa signal sequence, a 121aa mature region (aa18-138), and a 27aa C-terminal pro-peptide. There are two potential splice variants, one that shows a 48aa substitution, and another that shows a 33aa substitution for aa73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa18-138, human Ly6K shares 39% aa sequence identity with mouse Ly6K. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 10e6 cells using HeLa human cervical epithelial carcinoma cell line. Optimal dilutions to be determined by the researcher. Absorption: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice