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167676

LIN-28B (LIN-28 Homolog B, CSDD2, Lin-28.2)

Size

£843.00

SKU:
167676
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Sheep
Immunogen:
Recombinant protein corresponding to aa83-250 from human LIN-28B expressed in E.coli (Q6ZN17).
Format:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:
Lin­28B (Lineage protein 28 homolog B) is a 35­37kD member of the Lin28 family of proteins. Although there is an accompanying Lin­28/28A molecule, Lin­28 and Lin­28B are not alternative splice forms but represent products of separate genes. Lin­28B is found in both the cytoplasm and nucleus of fetal hematopoietic stem and progenitor cells, embryonic liver and multiple tumor types. It is considered a regulator of miRNA activity. Active miRNAs arise from transcribed RNAs that are first cleaved by nuclear RNase III, next cleaved by cytoplasmic Dicer, and finally associate with Argonaute to generate a RISC RNA silencing complex. Lin­28A participates in the cytoplasmic uridylation of miRNAs on their 3’­end, blocking Dicer processing and initiating RNA degradation. Lin­28B, by contrast, acts in the nucleus and blocks the initial RNase III cleavage step. Human Lin­28B is 250aa in length (SwissProt # Q6ZN17). It contains one CSD/cold shock domain (aa29­102) plus two CCHC­type Zn­finger RNA bindingdomains (aa127­144 and 149­166). Phosphorylation is known to occur on Ser105. There is one reported alternate start site at Met71, and there is a potential 11aa substitution for aa 1­4. Two isoforms of LIN28B with apparent MWs of 22kD and 37kD have been reported. Over aa83­250, human Lin­28B shares 84%aa sequence identity with mouse Lin­28B. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice