Leukemia Inhibitory Factor, Recombinant, Human, aa24-202 (LIF)

Size

£709.00

SKU:: 167675
Molecular Weight:: 19.6
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤0.01EU/1ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. No BSA added. Reconstitute with 250ul sterile PBS.
Extra Details:: Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. Recombinant protein corresponding to Pro24-Phe202 from human LIF, expressed in E. coli. Molecular Weight: ~19.6kD Biological Activity: Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is ~0.5ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.