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167655

Gi24 (VISTA, B7-H5, C10orf54, Dies1, Gi24, SISP) (PE)

Size

£863.00

SKU:
167655
Application:
Flow Cytometry
Purity:
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Recombinant protein corresponding to Phe33-Ala194 of human Gi24 expressed in NS0 cells.
Clone:
730804
Format:
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:
Platelet Receptor Gi24, also known as VISTA (V­domain Ig suppressor of T cell activation), B7­H5, B7H5, Dies1 (Differentiation of ESC­1), SISP1 and C10orf54, is a 55-65kD member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP­4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1­MMP expression and activity and serves as a substrate for MT1­MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V­type Ig­like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1­MMP, generating a soluble 30kD extracellular fragment plus a 25­30kD membrane­bound fragment. Over aa33­194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively. Applications: Suitable for use in Flow Cytometry. Optimal dilutions to be determined by the researcher. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in human peripheral blood mononuclear cells (PBMCs) Optimal dilutions to be determined by the researcher Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Shipping Conditions:
Blue Ice