Gi24 (VISTA, B7-H5, C10orf54, Dies1, Gi24, SISP) (APC)

Size

£889.00

SKU:: 167654
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Phe33-Ala194 from human Gi24, expressed in NS0 cells.
Clone:: 730804
Format:: Supplied as a liquid in 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), BH5, B7-H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65kD member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30kD extracellular fragment plus a 25-30kD membrane-bound fragment. Over aa33-194, human Gi24 shares 70% and 67%aa identity with mouse and rat Gi24, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 using human peripheral blood cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice