Axl (Axl Receptor Tyrosine Kinase, UFO) (APC)

Size

£889.00

SKU:: 167628
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aaMet1-Pro440 from human Axl expressed in NS0 cells.
Clone:: 108724
Format:: Supplied as a liquid in 1ml saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of receptor tyrosine kinases.1, 2. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887aa precursor that includes an 18aa signal sequence, a 426aa extracellular domain, a 21aa transmembrane segment, and a 422aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81%aa sequence identity. A short alternatively spliced form of human Axl is distinguished by a 9aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6), which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis 3. This family of receptor tyrosine kinases is involved in hematopoiesis, embryonic development, tumorigenesis, and the regulation of testicular functions. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice