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167622

AMBP (a1-Microglobulin, A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI)

Size

£825.00

SKU:
167622
Application:
WB
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Sheep
Immunogen:
Recombinant protein corresponding to aa20-203 from human a1­Microglobulin expressed in E.coli (P02760).
Format:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:
Human a1­Microglobulin (alpha1­m/A1M; also protein HC) is a secreted, 31­32kD glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. A1M appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. A1M circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human A1M is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP­1, a 120-140kD adrenomedullin­binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19aa signal sequence, an N­terminal 183aa A1M protein (aa20-203), and a C­terminal serine protease inhibitor termed bikunin (aa206­352). A1M possesses one lipocalin domain (aa42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31kD A1M and 28kD bikunin molecule, the 60­65kD AMBP precursor can also be released intact. A1M will undergo extracellular processing, generating a 30kD isoform that is missing aa199-203. There is one splice variant that shows a deletion of aa48­57. Over aa20­203, human A1M shares 76%aa sequence identity with both mouse and rat A1M. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice