VGlut3 (DFNA25, deafness autosomal dominant 25, SLC17A8, Solute carrier family 17 member 8)

Size

£626.00

SKU:: 167619
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGlut3.
Clone:: S34-34
Format:: Supplied as a liquid in PBS pH7.4, 50% glycerol.
Extra Details:: Vesicular Glutamate Transporter 3 (VGLUT3) is a multipass membrane protein restricted to synaptic vesicles of glutamergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: 1ug/ml was sufficient for detection of VGLut3 in 20ug of CV-1 fibroblast cells (lysate) transfected with VGlut3 by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.