167617
VGlut1 (BNPI, Slc17a7, Solute Carrier family 17 member 7, Vesicular glutamate transporter 1)
Size
£626.00
- SKU:
- 167617
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
- Clone:
- S28/9
- Format:
- Supplied as a liquid in PBS pH7.4, 50% glycerol.
- Extra Details:
- VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: 1ug/ml was sufficient for detection of VGLut1 in 20ug of rat brain lysate by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice