167604
SUR1 (ABC36, Abcc8, ATP binding cassette sub family C member 8, HHF1, HRINS, MRP8, PHHI, SUR)
Size
£626.00
- SKU:
- 167604
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
- Clone:
- S289-16
- Format:
- Supplied as a liquid in PBS pH7.4, 50% glycerol.
- Extra Details:
- Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: 1ug/ml was sufficient for detection of SUR1 in 20ug of mouse brain membrane lysate and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody. Western Blot: 1ug/ml Immunohistochemistry: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice