167601
Notch1 (Neurogenic locus notch homolog protein 1, hN1, Translocation associated notch protein, TAN-1, Motch A, mT14, p300 and Motch)
Size
£626.00
- SKU:
- 167601
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fusion protein amino acids 20-216(extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1, Accession# Q01705.
- Clone:
- S253-32
- Format:
- Supplied as a liquid in PBS pH7.4, 50% glycerol.
- Extra Details:
- Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Applications: Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: 1ug/ml was sufficient for detection of Notch1 in 20ug of rat brain membrane lysate and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody. Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice