Dicer (Dicer1, DCR, DCR-1, Double-strand-specific ribonuclease, Helicase with RNase motif, HERNA, Helicase MOI and KIAA0928)

Size

£626.00

SKU:: 167583
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer Accession# Q8R418.
Clone:: S167-7
Format:: Supplied as a liquid in PBS pH7.4, 50% glycerol.
Extra Details:: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3) Applications: Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: 1ug/ml was sufficient for detection of Dicer in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.