Clcn3 (chloride channel 3, chloride channel protein 3, CIC3)

Size

£626.00

SKU:: 167580
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3
Clone:: S258-5
Format:: Supplied as a liquid in PBS pH7.4, 50% glycerol.
Extra Details:: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: 1ug/ml was sufficient for detection of Clcn3 in 20ug of rat brain membrane lysate and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody. Western Blot: 1ug/ml Immunohistochemistry: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.