167578
Brevican (BCAN, BEHAB, CSPG7, Brain enriched hyaluronan binding protein antibody)
Size
£626.00
- SKU:
- 167578
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fusion protein amino acids 219-655 of rat Brevican
- Clone:
- S294A-6
- Format:
- Supplied as a liquid in PBS pH7.4, 50% glycerol.
- Extra Details:
- Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE Applications: Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: 1ug/ml was sufficient for detection of Brevican in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody. Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice