Cathepsin C (DPP1, Cathepsin J, CPPI, CTSC, dipeptidyl peptidase 1, dipeptidyl peptidase I, dipeptidyl transferase, dipeptidyl-peptidase I, DPP1, DPP-I, HMS, JP, JPD, PALS, PLS)

Size

£642.00

SKU:: 167362
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to aa238-252, C-RNVHGINFVSPVRNQ, from the internal region of human Cathepsin C (NP_001805.3).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase. Activates serine proteases such as elastase, cathepsin G and granzymes A and B. Can also activate neuraminidase and factor XIII. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Peptide): 1:128,000 (detection limit) Western Blot: 1-3ug/ml. ~24kD band observed in human neutrophil lysate (calculated MW of 51.8kD according to NP_001805.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice