157353
Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) Recombinant, Mouse
Size
£384.00
- SKU:
- 157353
- Molecular Weight:
- 53.1
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Extra Details:
- Recombinant protein corresponding to aa108-343 with a N-terminal His-tag and GST-tag from mouse MMTV expressed in E. coli. UniProt/Accession: P70701. Purity: ≥80% Form: Supplied as lyophilized powder in PBS, pH7.4, 5% sucrose, 0.01% sarcosyl. Reconstitute to a concentration of 0.1-0.5mg/ml in sterile dH2O, pH7.4. Do not vortex. Predicted Isoelectric Point: 9.1 Predicted Molecular Mass: 56kD Accurate Molecular Mass: 56kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Secreted, Extracellular matrix Amino Acid Sequence: SSL ETRNKVPYES PIFSRGFRES AFAYAIAAAG VVHAVSNACA LGKLKACGCD ASRRGDEEAF RRKLHRLQLD ALQRGKGLSH GVPEHPAILP ASPGLQDSWE WGGCSPDVGF GERFSKDFLD SREPHRDIHA RMRLHNNRVG RQAVMENMRR KCKCHGTSGS CQLKTCWQVT PEFRTVGALL RNRFHRATLIRPHNRNGGQL EPGPAGAPSP APGTPGLRRR ASHSDLVYFE KSP Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice