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157297

Vascular Cell Adhesion Molecule 1 (VCAM1) Recombinant, Rat

Size

£384.00

SKU:
157297
Molecular Weight:
35.8
Purity:
≥95%
Storage Conditions:
4°C/-70°C
Supplier:
United States Biological
Format:
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:
Source: Recombinant Rat from E. coli Accession No: P29534 Fragment: Thr369~Ala658 (Accession No: P29534) Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TL SPVGVEDEHS YLCTVTCQRR KLEKTIQVEV YSFPEDPEIE ISGPLVHGRP VTVNCTVPNV YPFDHLEIEL LKGETTLLNK FLREEIGTKS LETKSLEMTF IPTAEDTGKA LVCLAKLHSS QMESEPKQRQ STQTLYVNVA PKEPTIWVSP SPVPEEGSPV NLTCSSDGFP TPKILWSRQL KNGELQPLSQ NTTLSFMATK MEDSGIYVCE GINEAGISKK SVELIIQGSS KDIQLTVFPS KSVKEGDTVI ISCTCGSVPE IWIILKKKAK TGDMVLKSVN GSYTIRKA Epitope Tag: N-terminal Tags: His-tag and T7-tag Molecular Weight: 35.8kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Shipping Conditions:
Blue Ice