157182
Tryptase (TPS) Recombinant, Human
Size
£342.00
- SKU:
- 157182
- Molecular Weight:
- 43.1
- Purity:
- ≥90%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa161-262 from human TPS with N-Terminal tags: His-Tag and SUMO-tag. expressed in E. coli. UniProt/Accession: Q15661. Predicted Isoelectric Point: 6.3 Predicted Molecular Mass: 24.9kD Accurate Molecular Mass: 26kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Amino Acid Sequence: GDVDNDERLP PPFPLKQVKV PIMENHICDA KYHLGAYTGD DVRIVRDDML CAGNTRRDSC QGDSGGPLVC KVNGTWLQAG VVSWGEGCAQ PNRPGIYTRV TY Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice