Troponin I Type 3, Cardiac (TNNI3) Recombinant, Rat

Size

£412.00

SKU:: 157175
Molecular Weight:: 25.7
Purity:: ≥95%.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa1-211 with a His Tag at the N-Terminal of rat TNNI3 expressed in E. coli. Uniprot/Accession: P23693 Predicted Isoelectric Point: 9.5 Predicted Molecular Weight: 25.4kD Accurate Molecular Weight: 28kD as determined by SDS-PAGE reducing conditions Subcellular Location: Cytoplasm Amino Acid Sequence: MADESSDAAG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL KTLMLQIAKQ EMEREAEERR GEKGRVLSTR CQPLVLDGLG FEELQDLCRQ LHARVDKVDE ERYDVEAKVT KNITEIADLT QKIYDLRGKF KRPTLRRVRI SADAMMQALL GTRAKESLDL RAHLKQVKKE DIEKENREVG DWRKNIDALS GMEGRKKKFE G Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.