157133
Transforming Growth Factor Beta Induced Protein (TGFbI) Recombinant, Mouse
Size
£349.00
- SKU:
- 157133
- Molecular Weight:
- 26.9
- Purity:
- ≥95%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa423-632 with an N-terminal His-Tag from mouse Transforming Growth Factor Beta Induced Protein, expressed in E. coli. Swiss/UniProt Accession: P82198. Purity: ≥95% Predicted Isoelectric Point: 8.8 Predicted Molecular Weight: 29.6kD Accurate Molecular Weight: 28kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted, extracellular matrix Amino Acid Sequence: GVPRIDAQ MKTLLLNHMV KEQLASKYLY SGQTLDTLGG KKLRVFVYRN SLCIENSCIA AHDKRGRFGT LFTMDRMLTP PMGTVMDVLK GDNRFSMLVA AIQSAGLMEI LNREGVYTVF APTNEAFQAM PPEELNKLLA NAKELTNILK YHIGDEILVS GGIGALVRLK SLQGDKLEVS SKNNVVSVNK EPVAETDIMA TNGVVYAINT VL Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice