157101
Transferrin (TRF) Recombinant, Bovine
Size
£371.00
- SKU:
- 157101
- Molecular Weight:
- 36.1
- Purity:
- ≥95%
- Storage Conditions:
- 4°C/-70°C
- Supplier:
- United States Biological
- CAS Number:
- 11096-37-0
- Format:
- Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Source: Recombinant Bovine from E. coli Accession No: Q29443 Fragment: Asp284~Ala575 (Accession No: Q29443) Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-DVIWELL NHAQEHFGKD KPDNFQLFQS PHGKDLLFKD SADGFLKIPS KMDFELYLGY EYVTALQNLR ESKPPDSSKD ECMVKWCAIG HQERTKCDRW SGFSGGAIEC ETAENTEECI AKIMKGEADA MSLDGGYLYI AGKCGLVPVL AENYKTEGES CKNTPEKGYL AVAVVKTSDA NINWNNLKDK KSCHTAVDRT AGWNIPMGLL YSKINNCKFD EFFSAGCAPG SPRNSSLCAL CIGSEKGTGK ECVPNSNERY YGYTGAFRCL VEKGDVAFVK DQTVIQNTDG NNNEA Epitope Tag: N-terminal Tags: His-tag and T7-tag Molecular Weight: 36.1kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice