157096
Transcription Factor A, Mitochondrial (TFAM) Recombinant, Mouse
Size
£367.00
- SKU:
- 157096
- Molecular Weight:
- 50.6
- Purity:
- ≥90%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from 100mM sodium bicabonate, 500mM sodium chloride, pH 8.3, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin-300. Reconstitute with sterile dH2O to 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa43-243 with an N-terminal His and GST Tag from mouse TFAM, expressed in E. coli. Uniprot/Accession: P40630 Predicted Isoelectric Point: 9.1 Predicted Molecular Weight: 53.6kD Accurate Molecular Weight: 53kD as determined by SDS-PAGE, reducing conditions Subcellular Location: Mitochondrion Amino Acid Sequence: SSMGSYPK KPMSSYLRFS TEQLPKFKAK HPDAKLSELV RKIAALWREL PEAEKKVYEA DFKAEWKAYK EAVSKYKEQL TPSQLMGMEK EARQRRLKKK ALVKRRELIL LGKPKRPRSA YNIYVSESFQ EAKDDSAQGK LKLVNEAWKN LSPEEKQAYI QLAKDDRIRY DNEMKSWEEQ MAEVGRSDLI RRSVKRSGDI SEH Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice