Sulfatase 2 (SULF2) Recombinant, Human

Size

£384.00

SKU:: 156904
Molecular Weight:: 65
Purity:: ≥80%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa337-649 with an N-Terminal His and GST Tag, from human SULF2, expressed in E. coli. Uniprot/Accession: Q8IWU5 Amino Acid Sequence: FDIR VPFYVRGPNV EAGCLNPHIVLNIDLAPTIL DIAGLDIPAD MDGKSILKLL DTERPVNRFH LKKKMRVWRD SFLVERGKLLHKRDNDKVDA QEENFLPKYQ RVKDLCQRAE YQTACEQLGQ KWQCVEDATG KLKLHKCKGPMRLGGSRALS NLVPKYYGQG SEACTCDSGD YKLSLAGRRK KLFKKKYKAS YVRSRSIRSVAIEVDGRVYH VGLGDAAQPR NLTKRHWPGA PEDQDDKDGG DFSGTGGLPD YSAANPIKVTHRCYILENDT VQCDLDLYKS LQAWKDHKLH IDHEIETLQN KIKNLREVR Predicted Isoelectic Point: 8.7 Predicted Molecular Mass: 65.7kD Accurate Molecular Mass: 65kD as determined by SDS-PAGE reducing conditions Subcellular Location: Golgi Apparatus Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

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