Retinol Binding Protein 3, Interstitial (RBP3) Recombinant, Mouse

Size

£369.00

SKU:: 156635
Molecular Weight:: 34.5
Purity:: ≥95%. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -70°C/-20°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa18-320 with a N-terminal His-Tag from mouse RBP3 expressed in E. coli (P49194) Amino Acid Sequence: MGHHHHHHSGSEF-GPT HLFQPSLVLD MAKILLDNYC FPENLMGMQA AIEQAMKSHE ILGISDPQTL AQVLTAGVQS SLSDPRLFIS YEPSTLEAPQ QAPVLTNLTR EELLAQIQRN IRHEVLEGNV GYLRVDDLPG QEVLSELGEF LVSHVWRQLM GTSSLVLDLR HCSGGHFSGI PYVISYLHPG NTVMHVDTVY DRPSNTTTEI WTLPEVLGER YSADKDVVVL TSGHTGGVAE DIAYILKQMR RAIVVGERTE GGALDLQKLR IGQSNFFLTV PVSRSLGPLG GGGQTWEGSG VLPCVGTPAE QALEKALAIL Predicted Molecular Weight: 34.5kD Predicted Isoelectric Point: 5.4 Subcellular Location: Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD. No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice