156567
Pyruvate Kinase, Muscle (PKM2) Recombinant, Human
Size
£384.00
- SKU:
- 156567
- Molecular Weight:
- 51.2
- Purity:
- ≥95%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from PBS, pH7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with PBS, pH 7.4 to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa294-470 from human PKMS with an N-Terminal His and GST Tag, expressed in E. coli. Uniprot/Accession: P14618. Amino Acid Sequence: RGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF Predicted Isoelectric Point: 8.3 Predicted Molecular Mass: 49.3kD Accurate Molecular Mass: 50kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Nucleus, Cytoplasm Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice