Proteinase 3 (PR3) Recombinant, Mouse

Size

£400.00

SKU:: 156539
Molecular Weight:: 26.3
Purity:: ≥95%. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa24-249 with a N-Terminal His-Tag from mouse PR3 expressed in E. coli Accession No: Q61096 Amino Acid Sequence: MGHHHHHHSGSEF-AVQASKIVGG HEARPHSRPY VASLQLSRFP GSHFCGGTLI HPRFVLTAAH CLQDISWQLV TVVLGAHDLL SSEPEQQKFT ISQVFQNNYN PEENLNDVLL LQLNRTASLG KEVAVASLPQ QDQTLSQGTQ CLAMGWGRLG TQAPTPRVLQ ELNVTVVTFL CREHNVCTLV PRRAAGICFG DSGGPLICNG ILHGVDSFVI RECASLQFPD FFARVSMYVD WIQNVL Predicted Molecular Weight: 26.3kD Predicted Isoelectric Point: 6.5 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD. No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice