156511
Protein Kinase C Epsilon (PKCe), Recombinant, Human
Size
£390.00
- SKU:
- 156511
- Molecular Weight:
- 32.4
- Purity:
- ≥90%.
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% Sarcosyl, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to Leu403~Asp672 with N-Terminal His-Tag from human PKCe, expressed E. coli. Uniprot/Accession: Q02156. Amino Acid Sequence: LGLDEFNFIK VLGKGSFGKV MLAELKGKDE VYAVKVLKKD VILQDDDVDC TMTEKRILAL ARKHPYLTQL YCCFQTKDRL FFVMEYVNGG DLMFQIQRSR KFDEPRSRFY AAEVTSALMF LHQHGVIYRD LKLDNILLDA EGHCKLADFG MCKEGILNGV TTTTFCGTPD YIAPEILQEL EYGPSVDWWA LGVLMYEMMA GQPPFEADNE DDLFESILHD DVLYPVWLSK EAVSILKAFM TKNPHKRLGC VASQNGEDAI KQHPFFKEID Predicted Isoelectric Point: 5.4 Predicted Molecular Weight: 32.4kD Accurate Molecular Weight: 32.4kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice