Prominin 1 (PROM1) Recombinant, Human

Size

£411.00

SKU:: 156466
Molecular Weight:: 32.8
Purity:: ≥95%. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -70°C/-20°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 500mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.01% sodium azide. Reconstitute with sterile ddH2O.
Extra Details:: Recombinant protein corresponding to aa171-431 with two N-Terminal Tags, His-Tag and T-7Tag from human PROM1 expressed in E. coli (O43490) Predicted Molecular Weight: 32.8kD Predicted Isoelectric Point: 7.9 Subcellular Location: Cell projection, cilium, photoreceptor outer segment. Apical cell membrane; Multi-pass membrane protein Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM IGIFYGFVAN HQVRTRIKRS RKLADSNFKD LRTLLNETPE QIKYILAQYN TTKDKAFTDL NSINSVLGGG ILDRLRPNII PVLDEIKSMA TAIKETKEAL ENMNSTLKSL HQQSTQLSSS LTSVKTSLRS SLNDPLCLVH PSSETCNSIR LSLSQLNSNP ELRQLPPVDA ELDNVNNVLR TDLDGLVQQG YQSLNDIPDR VQRQTTTVVA GIKRVLNSIG SDIDNVTQRL PIQDILSAFS VYVNNTESYI HRNLPTLEEY D Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice