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156342

Pitrilysin Metalloproteinase 1 (MP1) Recombinant, Rat

Size

£356.00

SKU:
156342
Molecular Weight:
32.4
Purity:
≥95%
Storage Conditions:
4°C/-70°C
Supplier:
United States Biological
Format:
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:
Source: Recombinant Rat from E. coli Accession No: NP_001100833.1 Fragment: Asp653~Leu926 (Accession No: NP_001100833.1) Sequence: MGHHHHHHSG SEF-DLQETFSG MDQVKVMKRI AEMTDIKPIL RKLPRIKKYL LNCDNMRCSV NATPQQMPQA EKEVENFLRN VGRSKKERKP VRPHIVEKPT PSGPSGGAHA DGSQIIRKLI TDPTFKPCQM KTHFVLPFPV NYVGECVRTV PYADPDHASL KILARLMTAK FLHTEIREKG GAYGGGAKVT HTGIFTLYSY RDPNSIETLQ SFGKAIDWAK SGKFTQQDID EAKLSVFSAV DSPVAPSDKG MDHFLYGLSD EMKQTYREQL FAVTHDKLTS VSHKYL Epitope Tag: N-terminal His-Tag Molecular Weight: 32.4kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Shipping Conditions:
Blue Ice