Phospholipase A2, Lipoprotein Associated (LpPLA2) Recombinant, Mouse

Size

£364.00

SKU:: 156331
Molecular Weight:: 49.1
Purity:: ≥95%
Storage Conditions:: 4°C/-70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Lipoprotein-associated phospholipase A2 (Lp-PLA2) also known as platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene.[1][2] Lp-PLA2 is a 45kD protein of 441 amino acids.[3] It is one of several PAF acetylhydrolases. In the blood it travels mainly with low-density lipoprotein (LDL). Less than 20% is associated with high-density lipoprotein HDL. It is an enzyme produced by inflammatory cells and hydrolyzes oxidized phospholipids in LDL. Lp-PLA2 is platelet-activating factor (PAF) acetylhydrolase (EC 3.1.1.47), a secreted enzyme that catalyzes the degradation of PAF to inactive products by hydrolysis of the acetyl group at the sn-2 position, producing the biologically inactive products LYSO-PAF and acetate.[4] Lp-PLA2 is involved in the development of atherosclerosis,[3] an observation that has prompted interest as a possible therapeutic target (see, e.g. the investigational drug Darapladib). In human atherosclerotic lesions, 2 main sources of Lp-PLA2 can be identified, including that which is brought into the intima bound to LDL (from the circulation), and that which is synthesized de novo by plaque inflammatory cells (macrophages, T cells, mast cells). Source: Recombinant Mouse from E. coli Accession No: Q60963 Fragment: Phe22~Asn440 (Accession No: Q60963) Sequence: MGHHHHHHSG SEFELRRQ-FH WQDTSSFDFR PSVMFHKLQS VMSAAGSGHS KIPKGNGSYP VGCTDLMFGY GNESVFVRLY YPAQDQGRLD TVWIPNKEYF LGLSIFLGTP SIVGNILHLL YGSLTTPASW NSPLRTGEKY PLIVFSHGLG AFRTIYSAIG IGLASNGFIV ATVEHRDRSA SATYFFEDQV AAKVENRSWL YLRKVKQEES ESVRKEQVQQ RAIECSRALS AILDIEHGDP KENVLGSAFD MKQLKDAIDE TKIALMGHSF GGATVLQALS EDQRFRCGVA LDPWMYPVNE ELYSRTLQPL LFINSAKFQT PKDIAKMKKF YQPDKERKMI TIKGSVHQNF DDFTFVTGKI IGNKLTLKGE IDSRVAIDLT NKASMAFLQK HLGLQKDFDQ WDPLVEGDDE NLIPGSPFDA VTQVPAQQHS PGSQTQN Epitope Tag: N-terminal His-Tag Molecular Weight: 49.1kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice