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156296

Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) Recombinant, Mouse

Size

£349.00

SKU:
156296
Molecular Weight:
24.7
Purity:
≥95%
Storage Conditions:
-70°C
Supplier:
United States Biological
Format:
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:
Recombinant protein corresponding to Cys577-Asn766 with an N-terminal His-Tag from mouse, expressed in E. coli. Swiss/UniProt Accession: O70343. Amino Acid Sequence: CSRS PYSRSRSRSP GSRSSSRSCY YYESSHYRHR THRNSPLYVR SRSRSPYSRR PRYDSYEAYE HERLKRDEYR KEHEKRESER AKQRERQKQK AIEERRVIYV GKIRPDTTRT ELRDRFEVFG EIEECTVNLR DDGDSYGFIT YRYTCDAFAA LENGYTLRRS NETDFELYFC GRKQFFKSNY ADLDTN Predicted Isoelectric Point: 9.4 Predicted Molecular Weight: 24.7kD Accurate Molecular Mass: 24kD as determined by SDS-PAGE under reducing conditions. Subcellular Location: Nucleus Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Shipping Conditions:
Blue Ice