Perforin 1 (PRF1) Recombinant, Mouse

Size

£368.00

SKU:: 156257
Molecular Weight:: 65
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa40-355 with a N-terminal His and GST tag from mouse Perforin 1, expressed in E. coli. Swiss/Uniprot Number: P10820. Amino Acid Sequence: V WMAGEGMDVT TLRRSGSFPV NTQRFLRPDR TCTLCKNSLM RDATQRLPVA ITHWRPHSSH CQRNVAAAKV HSTEGVAREA AANINNDWRV GLDVNPRPEA NMRASVAGSH SKVANFAAEK TYQDQYNFNS DTVECRMYSF RLVQKPPLHL DFKKALRALP RNFNSSTEHA YHRLISSYGT HFITAVDLGG RISVLTALRT CQLTLNGLTA DEVGDCLNVE AQVSIGAQAS VSSEYKACEE KKKQHKMATS FHQTYRERHV EVLGGPLDST HDLLFGNQAT PEQFSTWTAS LPSNPGLVDY SLEPLHTLLE EQNPK Predicted Isoelectric Point: 7.4 Predicted Molecular Mass: 67.2kD Accurate Molecular Mass: 65kD as determined by SDS-PAGE reducing conditions Subcellular Localization: Membrane, Secrested, Cytoplasm. Endosome Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

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