Peptidase Inhibitor 16 (PI16) Recombinant, Mouse

Size

£327.00

SKU:: 156243
Molecular Weight:: 75
Purity:: ≥80%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl,, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-0.25mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to Leu21-Lys468 with N-Terminal His Tag from mouse PI16, expressed in E. coli Swiss/UniProt Accession: E9QNE5. Amino Acid Sequence: LIATGPTTAL TEDEKQTMVD LHNQYRAQVS PPASDMLQMR WDDELAAFAK AYAQKCVWGH NKERGRRGEN LFAITDEGMD VPLAVGNWHE EHEYYNFSTA TCDPNQMCGH YTQVVWSKTE RIGCGSHFCE TLQGVEEANI HLLVCNYEPP GNVKGRKPYQ EGTPCSQCPL GYSCENSLCE PMRNPEKAQD SPPRVTEVPS TRATEAPSSR ETGTPSLATS ETLHFSVTKV SDSLATESSP AVETKAPSSL ATEGPSSMAT EAQAFVTEVP LVSARHMQPS VDEGPVNFLT STHIPVPKSM DEEASKSSAT SVSPKKSLYP KMSLTESGES VPQIQEEAEP KDELSEPEAI LPEAEAAPTE AEVELREPEA ESPKAESPEA EAESPLSSEA LVPVLPAQER GGQKASLEHS GHPASPSLPT FPSASGNATG GRTLALQSSW TGAENPEK Predicted Isoelectric Point: 4.4 Predicted Molecular Weight: 54.9kD Accurate Molecular Weight: 75kD as determined by SDS-PAGE reducing conditions Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice