156151
Nucleolin (NCL) Recombinant, Human
Size
£362.00
- SKU:
- 156151
- Molecular Weight:
- 24.3
- Purity:
- ≥90%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder in PBS, pH7.4, 5% trehalose, 0.01% sarcosyl. Reconstitute in sterile 10mM PBS, pH7.4 to a concentration of 0.1-1.0mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa388-569 from human NCL with N-Terminal His-Tag expressed in E. coli. UniProt/Accession: P19338. Predicted Isoelectric Point: 6.7 Predicted Molecular Mass: 24.3kD Accurate Molecular Mass: 24kD as determined by SDS-PAGE reducing conditions Subcellular Location: Nucleus, Cytoplasm Amino Acid Sequence: KER DARTLLAKNL PYKVTQDELK EVFEDAAEIR LVSKDGKSKG IAYIEFKTEA DAEKTFEEKQ GTEIDGRSIS LYYTGEKGQN QDYRGGKNST WSGESKTLVL SNLSYSATEE TLQEVFEKAT FIKVPQNQNG KSKGYAFIEF ASFEDAKEAL NSCNKREIEG RAIRLELQGP RGSPNARSQ Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 10mM PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice