155838
Matrix Metalloproteinase 9 (MMP9) Recombinant, Mouse
Size
£351.00
- SKU:
- 155838
- Molecular Weight:
- 19.9
- Purity:
- ≥95%.
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 5% Proclin300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa225-390 with an N-Terminal His-Tag from mouse MMP9 expressed in E. coli Swiss/UniProt Accession: P41245. Predicted Isoelectric Point: 6.2 Predicted Molecular Weight: 19.6kD Accurate Molecular Mass: 20kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Secreted, extracellular matrix Amino Acid Sequence: SNGAPC HFPFTFEGRS YSACTTDGRN DGTPWCSTTA DYDKDGKFGF CPSERLYTEH GNGEGKPCVF PFIFEGRSYS ACTTKGRSDG YRWCATTANY DQDKLYGFCP TRVDATVVGG NSAGELCVFP FVFLGKQYSS CTSDGRRDGR LWCATTSNFD TDKKWGFCPD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice