Interferon Beta (IFNb) Recombinant, Rabbit

Size

£384.00

SKU:: 155281
Molecular Weight:: 22.9
Purity:: ≥95%; Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -70°C/-20°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein correspondint to aa22-180 with two N-Terminal Tags, His-Tag and T7-Tag from rabbit IFNb, expressed in E. coli (G1TTX4) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MSYNSLQIQ LWHGSLTCAK LLLQLNGTTE DCLNERINFK VPKEIKEPQQ LQKEDTTLVI FEMLNNIFDI FRKNFSSTGW NETLVENLLG ETHLQIHHLK SKINKKVTLE SIRMNLRLKS YYWRIMDYLE TKQYSNCAWK IVQLEIFRNF SFIIMLIDYL Predicted Molecular Weight: 22.9kD Predicted Isoelectric Point: 8.7 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use. Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD. Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier. Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel: Mini gels: 3-5ul/well Large gel: 7ul/well The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice