Insulin Like Growth Factor Binding Protein 5 (IGFBP5) Recombinant, Rat

Size

£346.00

SKU:: 155202
Molecular Weight:: 29.2
Purity:: ≥95%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconsitute with sterile dH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to His25-Glu271 of rat IGFBP5 with a N-terminal His-Tag expressed in E. coli. (P24594) Amino Acid Sequence: HCEPCD EKALSMCPPS PLGCELVKEP GCGCCMTCAL AEGQSCGVYT ERCAQGLRCL PRQDEEKPLH ALLHGRGVCL NEKSYGEQTK IERDSREHEE PTTSEMAEET YSPKVFRPKH TRISELKAEA VKKDRRKKLT QSKFVGGAEN TAHPRVIPAP EMRQESDQGP CRRHMEASLQ EFKASPRMVP RAVYLPNCDR KGFYKRKQCK PSRGRKRGIC WCVDKYGMKL PGMEYVDGDF QCHAFDSSNV E Predicted Isoelectric Point: 8.5 Predicted Molecular Weight: 29.2kD Subcellular Location: Secreted Accurate Molecular Mass: 35kD as determined by SDS-PAGE reducing conditions Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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