154893
Granzyme K (GZMK) Recombinant, Human
Size
£322.00
- SKU:
- 154893
- Molecular Weight:
- 27.1
- Purity:
- ≥95%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin-500 . Reconstitute with sterile PBS to a concentration of 0.1-0.5mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa27-264 with an N-Terminal Hs-Tg from human GZMK, expressed in E. coli. Uniprot/Accession: P49863 Amino Acid Sequence: IIGG KEVSPHSRPF MASIQYGGHH VCGGVLIDPQ WVLTAAHCQY RFTKGQSPTV VLGAHSLSKN EASKQTLEIK KFIPFSRVTS DPQSNDIMLV KLQTAAKLNK HVKMLHIRSK TSLRSGTKCK VTGWGATDPD SLRPSDTLRE VTVTVLSRKL GIYTLLTKKY QTWIKSNLVP PHTN Predicted Isoelectric Point: 9.5 Predicted Molecular Weight: 27.1kD Accurate Molecular Mass: 30kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Secreted, Cytoplasm Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice