Galectin 12 (GAL12) Recombinant, Human

Size

£371.00

SKU:: 154711
Molecular Weight:: 29.2
Purity:: ≥95%. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: 4°C/-70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corressponding to aa70-291 of human Galectin 12 expressed in E. coli with two N-terminal Tags, His-tag and T7-tag (Q96DT0) Predicted Molecular Weight: 29.2kD Subcellular Location: Nucleus Predicted isoelectric Point: 9.7 Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- P LDAHRFQVDF QCGCSLCPRP DIAFHFNPRF HTTKPHVICN TLHGGRWQRE ARWPHLALRR GSSFLILFLF GNEEVKVSVN GQHFLHFRYR LPLSHVDTLG IFGDILVEAV GFLNINPFVE GSREYPAGHP FLLMSPRLEV PCSHALPQGL SPGQVIIVRG LVLQEPKHFT VSLRDQAAHA PVTLRASFAD RTLAWISRWG QKKLISAPFL FYPQRFFEVL L Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stabiblity: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice