FK506 Binding Protein 10 (FKBP10) Recombinant, Mouse

Size

£371.00

SKU:: 154644
Molecular Weight:: 29.7
Purity:: ≥95%.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, ,5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa316-573 with an N-Terminal His Tag from mouse FKBP10, expressed in E. coli. Uniprot/Accession: Q61576 Amino Acid Sequence: VGQGY IIPGMDQGLQ GACIGERRRI TVPPHLAYGE NGTGDKIPGS AVLIFDVHVI DFHNPSDPVE IKTLSRPPEN CNETSKIGDF IRYHYNCSLL DGTRLFSSHD YEAPQEITLG ANKVIEGLDR GLQGMCVGER RQLIVPPHLA HGENGARGVP GSAVLLFEVE LVSREDGLPT GYLFVWYQDP STSLFEDMDL NKDGEVPPEE FSSFIKAQVN EGKGRLMPGQ DPDKTISDMF QNQDRNQDGK ITAEELKLKS DED Predicted Isoelectric Point: 5.0 Predicted Molecular Weight: 29.7kD Accurate Molecular Weight: 30kD as determined by SDS-PAGE reducing conditions Subcellular Location: Endoplasmic reticulum lumen Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

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