154423
Endothelin 1 (EDN1) Recombinant, Human
Size
£364.00
- SKU:
- 154423
- Molecular Weight:
- 6
- Purity:
- ≥90%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from PBS pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding aa53-90 with an N-terminal His tag from human Endothelin 1, expressed in E. coli. Uniprot/Acccession: P05305. Amino Acid Sequence: CSCSSLMD KECVYFCHLD IIWVNTPEHV VPYGLGSPRS Predicted Isoelectric Point: 6.2 Molecular Weight: 5.5kD Accurate Molecular Mass: 6kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice