154098
Collagen Type VII (COL7) Recombinant, Human
Size
£335.00
- SKU:
- 154098
- Molecular Weight:
- 32.2
- Purity:
- ≥95%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder in PBS, pH7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile dH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa190-472 of human COL7with N-Terminal His-Tag expressed in E. coli. Uniprot/Acession: Q02388. Predicted Isoelectric Point: 6.4 Predicted Molecular Mass: 31.9kD Accurate Molecular Mass: 34kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Secreted, extracellular matrix Amino Acid Sequence: P TSDFFFFVND FSILRTLLPL VSRRVCTTAG GVPVTRPPDD STSAPRDLVL SEPSSQSLRV QWTAASGPVT GYKVQYTPLT GLGQPLPSER QEVNVPAGET SVRLRGLRPL TEYQVTVIAL YANSIGEAVS GTARTTALEG PELTIQNTTA HSLLVAWRSV PGATGYRVTW RVLSGGPTQQ QELGPGQGSV LLRDLEPGTD YEVTVSTLFG RSVGPATSLM ARTDASVEQT LRPVILGPTS ILLSWNLVPE ARGYRLEWRR ETGLEPPQKV VLPSDVTRYQ LD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice