154095
Collagen Type VI Alpha 1 (COL6a1) Recombinant, Mouse
Size
£377.00
- SKU:
- 154095
- Molecular Weight:
- 21.5
- Purity:
- ≥95%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from PBS, pH 7.4, 5% trehalose and 0.01% sarcosyl. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa816-1002 with an N-Terminal Hos-Tag from mouse COL6a1, expressed in E. coli. Uniprot/Accession: Q04857 Amino Acid Sequence: TCPIT FSSPADITIL LDSSASVGSH NFETTKVFAK RLAERFLSAG RADPSQDVRV AVVQYSGQGQ QQPGRAALQF LQNYTVLASS VDSMDFINDA TDVNDALSYV TRFYREASSG ATKKRVLLFS DGNSQGATAE AIEKAVQEAQ RAGIEIFVVV VGPQVNEPHI RVLVTGKTAE YDVAFGERHL FR Predicted Isoelectric Point: 6.1 Predicted Molecular Weight: 21.5kD Accurate Moleculor Mass: 22kD as determinbed by SDS-PAGE reducing conditions Subcellular Location: Secreted, extracellular matrix Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice