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153962

Charcot Leyden Crystal Protein (CLC) Recombinant, Human

Size

£368.00

SKU:
153962
Molecular Weight:
17.6
Purity:
≥90%
Storage Conditions:
-70°C
Supplier:
United States Biological
Format:
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to 0.1-1mg/ml. Do not vortex.
Extra Details:
Recombinant protein corresponding to Ser2-Arg142 with an N-Terminal His-Tag from human CLC, expressed in E. coli Swiss/UniProt Accession: Q05315. Amino Acid Sequence: SLLPVPYTEA ASLSTGSTVT IKGRPLACFL NEPYLQVDFH TEMKEESDIV FHFQVCFGRR VVMNSREYGA WKQQVESKNM PFQDGQEFEL SISVLPDKYQ VMVNGQSSYT FDHRIKPEAV KMVQVWRDIS LTKFNVSYLK R Predicted Isoelectric Point: 6.8 Predicted Molecular Weight: 17.6kD Accurate Molecular Mass: 16kD as determined by SDS-PAGE under reducing conditions Subcellular Location: Cytoplasm Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Shipping Conditions:
Blue Ice