153740
C Reactive Protein (CRP) Recombinant, Porcine
Size
£327.00
- SKU:
- 153740
- Molecular Weight:
- 24.1
- Purity:
- ≥95%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, Proclin300. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa20~222 with an N-Terminal His-Tag from Porcine, expressed in E. coli Swiss/Uniprot Accession: O19062. AA Sequence: MGHHHHHHSGSEF-Q TDMIGKAFVF PKESENSYVS LTARLTKPLT AFTVCLRVYT DLNRDYSLFS YATKTQYNEI LLFRGKTAVY SISVGGADVV FKPHQSSEPM HFCMTWESTS GITELWVDGK PMVRRSLKRG YSLGTQASII LGQEQDAFAG GFEKNQCLVG DIGDVNMWDY VLSPEEINTV YAGGTFSPNV LNWRALRYEM SGEVYVKPQL WP Predicted Isoelectric Point: 9.1 Predicted Molecular Weight: 24.4kD Accurate Molecular Mass: 25kD as determined by SDS-PAGE under reducing conditions. Subcellar Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice