Brain Derived Neurotrophic Factor (BDNF) Recombinant, Human

Size

£420.00

SKU:: 153723
Molecular Weight:: 40
Purity:: ≥95%.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with 20mM Tris, 150mM sodium chloride to a concentration of 0.1-1.0mg-ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa129-247 with an N-Terminal His-Tag from human BDNF expressed in E. coli Swiss/UniProt Accession: P23560. Amino Acid Sequence: HS DPARRGELSV CDSISEWVTA ADKKTAVDMS GGTVTVLEKV PVSKGQLKQY FYETKCNPMG YTKEGCRGID KRHWNSQCRT TQSYVRALTM DSKKRIGWRF IRIDTSCVCT LTIKRGR Predicted Molecular Weight: ~16.7kD Predicted Isoelectric Point: 8.5 Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 20mM Tris, 150mM sodium chloride. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice